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KMID : 0381920060360040271
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Korean Journal of Microscopy
2006 Volume.36 No. 4 p.271 ~ p.277
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Ultrastructures of Zinc-containing Mast Cells Found in the Rat Hindpaw after an Inflammatory Stimuli: Zinc Selenium Autometallography
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Lee Bo-Ye
Kim Yi-Suk
Lee Beob-Yi
Lee Hyun-Suk
Tack Gye-Rae
Lee Young-Il
Lee Jung-Yeol
Jo Seung-Mook
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Abstract
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Mast cells (MCs) are granulated cells that play a pivotal role in allergic reaction and inflammation. The granules of mast cells are known to be rich in zinc (Zn). Male Sprague-Dawley rats were used. We injected 200 μL of complete Freund’s adjuvant (CFA) subcutaneously in the dorsal aspect of one hindpaw. Finally, zinc selenium autometallography (AMG) was done by Danscher’s method. The present study showed the ultrastructures of zinc-containing mast cells found in inflammatory area following an complete freund’s adjuvant (CFA) inoculation into the rat hindpaw.At light microscopic level, mast cells were round or oval, at average 12 μm in diameter, with many filopodia extending from the cell surface. Because the rather small and spherical nucleus was centrally placed; it was frequently obscured by the cytoplasmic granules, it sometimes could not be seen. Mast cells were distributed chiefly in the vicinity of small blood vessels. In most preparation many mast cells were ruptured and their granules escaped into the surrounding tissue.In electron micrographs, The secretory granules were at average 0.5 μm in diameter and were limited by a membrane. The cell surface contained numerous microvilli and folds. Their interior was heterogenous in appearance. The nucleus was surrounded by large numbers of prominent vesicels and a well developed Golgi apparatus, but scant endoplasmic reticulum.
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KEYWORD
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CFA, AMG, Zinc, Pain, Mast cells, Hindpaw, Rat
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